Transformation of an artificial microRNA to soybean (Glycine max (L.) Merr.) to reduce the parasitism of Meloidogyne incognita

Effectors are specific proteins secreted by nematodes into plant cells that facilitate their parasitism to host plants. Inactivation of these effectors could reduce the parasitic ability on plants and damage caused nematodes. Gene Minc14137encodes an effector unknown function is cloned from Meloidogyne incognita. Artificial microRNAs capable inactivating gene Minc14137 were synthesised inserted expression vector in soybean. This construct was transformed soybean cotyledon node mediated Agrobacterium tumefaciens regenerated transgenic Copy number level miRNA T0 determined qPCR technique. In T1 plants, pathogenic root-knot nematode reduced 44.6-50.5% compared control Results show MINC14137 play important role